Leukocytes Profil of Monopterus albus raired in Biofloc System with Different Stock Densities
DOI:
https://doi.org/10.31258/jas.5.2.137-145Keywords:
Monopterus albus, Leukocytes, Phagocyte, Lymphocytes, MonocytesAbstract
Biofloc is an agglomerate composed of various autotrophic and heterotrophic organisms as well as particles, which are mixed by aeration and circulation to form clumps that integrate quite well in water. This research was carried out in March- May 2021 in the Hatcheries and Experimental Ponds and Parasites and Fish Diseases Laboratory, Faculty of Fisheries and Marine, Universitas Riau. The study aimed to analyze the leukocyte profile of the Monopterus albus raired biofloc system with different stock densities. The research method used a one-factor, completely randomized design (CRD) with four treatments with three replications. The treatments applied were P1 (of 10 fish/25 L), P2 (of 20 fish/25 L), P3 (of 30 fish/25 L), and P4 (of 40 fish/25 L). M. albus were reared in a biofloc system for 40 days. The results showed that there was an effect of stock densities on the leukocyte profile of M. albus reared in biofloc systems with different stock densities, a significant effect (P<0.05). The best stock density was at P3 (30 fish/25 L), where the total leukocytes were 3.62 x 104 cells/mm3, leucocrite was 2.00%, phagocyte activity was 36.66%, while the percentage of lymphocytes was 83.00 %, monocytes was 16.00%, and neutrophils was 13.00%. Water quality during this study was at temperature 26-300C, pH 6-7, dissolved oxygen 4.4-6.8 mg/L, and ammonia 0.02-0.06 mg/L. It was concluded that there was an effect on the biofloc system of stock density on the leucocyte profile M. albus
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References
Anderson, D.P., and A.K Siwicki. 1995. Basic Hematology and Serology for Fish Health Programs. Fish Health Section, Asian Fisheries Society.
Astawan, M. 2008. Sehat dengan Hidangan Hewani. Penebar Swadaya. Jakarta.
Avnimelech, Y., 2009. Bio-filters: The Need for an New Comprehensive Approach. Aquaculture Engineering, 34: 172-178.
Hartika, R., Mustahal, M., dan Putra, A.N. 2014. Gambaran Darah Ikan Nila (Oreochromis niloticus) dengan Penambahan Dosis Prebiotik yang berdeda dalam Pakan. Jurnal Perikanan dan Kelautan, 4(4): 259 - 267
Hidayaturrahman, H., Muhamat, A., dan Nurliani, N. 2013. Profil Fisiologi Darah Ikan Nila (Oreochromis niloticus L) pada Budidaya Keramba di Sungai Riam Kanan. Jurnal Bioscientiae 10(1): 101 – 109
Iman, K.N. 2017. Diferensiasi Leukosit ikan Jambal Siam (Pangasius hypopthalmus) yang diberi Pakan Mengandung Ekstrak Kurkumin Kunyit (Curcuma domestica V). Fakultas Perikanan dan Kelautan. Universitas Riau.
Lukistyowati, I. 2011. Efektifitas Bawang Putih (Allium sativum L) untuk Meningkatkan Ketahanan Tubuh Ikan Mas (Cyprinus carpio L) terhadap Penyakit Motile Aeromonas Septicemia. Program Doktor Sain Veteriner. Fakultas Kedokteran Hewan Universitas Gadjah Mada
Puspasari, K. 2013 Karakteristik Protein Antigenik Larva Gnathostoma spinigerum pada Ikan Belut Rawa (Monopterus alba) menggunakan Teknik Immunoglotting. Institut Pertanian Bogor. Bogor.
Putra, I., Rusliadi, R., and Fauzi, F. 2017 Growth Performance and Feed Untilization of African catfish Clarias Gariepinus Fed a Commercial Diet Reared the Biofloc System Enchaced with Probiotic. F1000research
Purwanto, A. 2006. Gambaran Darah Ikan Mas (Cyprinus carpio) yang Terinfeksi Koi Herpes Virus. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor. Bogor.
Septiarini, S., Harpeni, E., dan Wardiyanto, W. 2012. Pengaruh Waktu Pemberian Probiotik yang Berbeda terhadap Respon Imun Non–Spesifik Ikan Gurami (Osphronemus gouramy L.) yang Diuji Tantang dengan Bakteri Aeromonas salmonicida. Jurnal Rekayasa dan Teknologi Budidaya Perairan, 1(1): 1-8.
Simanullang, D.F.P. 2017. Pengaruh Penambahan Sumber Karbon yang Berbeda pada sistem Bioflok terhadap Laju Pertumbuhan dan Kelulushidupan Ikan Nila Merah (Oreochromis niloticus). Fakultas Perikanan dan Kelautan. Universitas Riau.
Syatma, M. 2016 Penambahan Simplisia Kulit Buah Manggis (Grnicia mangostana) dalam pakan terhadap diferensiasi leukosit ikan lele dumbo (Clarias gariepinus) yang diinfeksi Aeromonas hydrophyla. Unversitas Riau Pekanbaru
Utami, D.T., Prayitno, S.B., Hastuti, S., dan Santika, A. 2013. Gambaran Parameter Hematologis pada Ikan Nila (Oreochromis niloticus) yang Diberi Vaksin DNA Streptococcus iniae dengan Dosis yang Berbeda. Journal of Aquaculture Management and Technology, 2 (4) : 7-20
Widanarni, W. 2018. Budidaya berbasis Mikroba untuk Akuakultur Berkelanjutan. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor. 87 hlm.
Windarti, W., Pulungan, C., Pamukas, N.A., Riauwaty, M., Asiah, N., dan Heltonika, B. 2015. Buku Ajar Fisiologi Hewan Air. Ur Prees. Riau. Pekanbaru. 114 hlm
